Technical Officer for The Protein Expression Core - Barcelona, España - IRB Barcelona

IRB Barcelona
IRB Barcelona
Empresa verificada
Barcelona, España

hace 1 mes

Isabel García

Publicado por:

Isabel García

beBee Recruiter
Descripción

Created in 2005 by the Generalitat de Catalunya (Government of Catalonia) and the University of Barcelona, IRB Barcelona is a Severo Ochoa Centre of Excellence—a seal that was awarded in 2011.


The institute is devoted to conducting research of excellence in biomedicine and to transferring results to clinical practice, thus improving people's quality of life, while simultaneously promoting the training of outstanding researchers, technology transfer, and public communication of science.

Its 28 laboratories and seven core facilities address basic questions in biology and are orientated to diseases such as cancer, metastasis, Alzheimer's, diabetes, and rare conditions.

IRB Barcelona is an international centre that hosts 400 members and 35 nationalities. It is located in the Barcelona Science Park.

IRB Barcelona forms part of the Barcelona Institute of Science and Technology (BIST) and the "Xarxa de Centres de Recerca de Catalunya" (CERCA).

Under the INVESTIGO program, IRB Barcelona is seeking a
Technical Officer for the Protein Expression Core Facility.

DUTIES:


The primary duties will be the maintenance of our suspension eukaryotic cell lines for protein production purposes, transfection of cell lines to produce protein or recombinant baculoviruses and amplification of baculoviruses.


Secondary duties may include cloning of genes into expression or shuttle plasmids, small scale purifications/expression screenings, purification of proteins from transfected/baculo-infected cell lines and maintaining stocks of all necessary reagents and media.


SELECTION CRITERIA:


  • Not having previous labour relations with the IRB Barcelona within a period of 6 months, before the beginning of the contract.
  • Availability to join the IRB Barcelona by the 15th of October 2023.

EXPERIENCE, KNOWLEDGE, SKILLS:


Must Have - Required:

-
Experience: Extensive tissue culture experience (cell lines), excellent sterile technique. Molecular Biology experience.
-
Knowledge:All aspects of gene cloning (PCR, clone identification, sequence confirmation etc.), plasmid preparation.
-
Skills:Sterile technique. Handling and transfection of litre-scale eukaryotic suspension cultures. Cryopreservation of cell stocks. Competent molecular biologist. Excellent record keeping skills.


Desirable:

-
Experience: Some experience in protein purification would be advantageous as would previous work with baculovirus expression systems.
-
Knowledge: Understanding of protein purification methodologies-affinity methods, size exclusion, ion exchange etc.
-
Skills: Hands-on protein purification experience using FPLC instrumentation would be an advantage.


WORKING CONDITIONS & ENTITLEMENTS:

-
Working conditions: two year contract linked to the INVESTIGO Programme, to allocate young people in search of a job in the research field, under the framework project: "Pla de Recuperació, Transformació i Resiliència". Employed in compliance with Spanish legislation and regulations under a full-time contract. Employees receive the benefits of the Spanish Social Security system covering sickness, maternity/paternity leave and injuries at work.
-
Estimated annual gross salary:24.875€.
Salary commensurate with experience and qualifications.
-
International Environment:The opportunity to join a prestigious, diverse and inclusive international research institution.
-
Benefits: Continuous training in a high-quality environment with unique professional career opportunities.


HOW TO APPLY & SELECTION PROCESS:
Applications for the above opening should include a Full CV, in the Anonym Curriculum Vitae format where name, age, gender and any other personal reference, are not included
-
Number of positions available: 1

-
Selection process:

-
Pre-selection: Will be based on CV, experience and required conditions met from the INVESTIGO Programme.
Más ofertas de trabajo de IRB Barcelona
Ver todas las ofertas de empleo de IRB Barcelona