Bioinformatician or Wet Lab Predoctoral Position to - Barcelona, España - Somm Excellence Alliance

Somm Excellence Alliance

Empresa verificada

Barcelona, España

hace 3 semanas

Publicado por:

Isabel García

beBee Recruiter

Descripción

The Barcelona Institute for Global Health (ISGlobal) is a cutting-edge institute addressing global public health challenges through research, translation into policy and education.

ISGlobal has a broad research portfolio in communicable and non-communicable diseases, ranging from basic research at the molecular level to studies at the population level.

ISGlobal is accredited with the Severo Ochoa distinction, a seal of excellence of the Spanish Science Ministry.

Venue

Barcelona

What We Are Looking for

Wet lab profiles

Bioinformatician profiles

The team

The ISGlobal Malaria Epigenetics lab directed by ICREA Research Professor Alfred Cortés investigates the regulation of gene expression in the malaria parasite Plasmodium falciparum.

We use a combination of approaches that include genome editing with CRISPR-Cas9 technology, state-of-the-art phenotypic characterisation and multi-omic approaches. Our ultimate goal is to generate high-quality basic knowledge that is relevant for the fight against malaria.

About the project

Plasmodium falciparum is responsible for the most severe forms of human malaria.

Transcriptional regulation plays a key role in malaria parasite biology, as it controls fundamental processes such as life cycle progression, antigenic variation and adaptation to the conditions of the environment.

Some of these processes are regulated at the epigenetic level, with a key role for euchromatin-heterochromatin transitions.

Of note, heterochromatin plays a prominent role in the regulation of the conversion of some asexual parasites into sexual forms that mediate transmission to mosquito vectors.

Another important process regulated at the transcriptional level is the heat-shock response, which enables parasite survival during malaria febrile episodes.

Wet lab profiles. On the day to day, the main experimental techniques used for this project will include culture and manipulation of P. falciparum (including genome editing with CRISPR/Cas9), stateoftheart transcriptomic and epigenomic analyses, and advanced phenotypic characterisation using functional assays, among many others. In addition, this project will involve establishing and optimising in our laboratory several new techniques, including CUT&TAG, Hi-C / UMI4C, singlecell omics and proximity labelling, with support from our collaborators.
Bioinformatician profiles. As part of this project, we will generate a large amount of multiomic data. The bioinformatician will use bioinformatic tools to analyse and integrate large multiomic datasets generated by other members of the team. While we have already established robust pipelines for the analysis of P. falciparum RNAseq and ChIPseq data, new pipelines for the analysis of CUT&TAG, Hi-C / UMI4C and singlecell omics data will have to be developed. Novel, creative approaches will be needed for the analysis of some complex datasets. The bioinformatician will also work on the integration of the different omic datasets, and use previously published datasets available in public repositories. We consider very important to establish a fluent communication between bioinformaticians and wet lab scientists.

Deadline:
December 31, 2023

Job category:
PhD Student

Area:
Life Sciences