Technician - Study of Lymphoma Formation - Barcelona, España - Center for Genomic Regulation

Center for Genomic Regulation

Empresa verificada

Barcelona, España

hace 3 semanas

Publicado por:

Isabel García

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Descripción

The Institute

The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries.

The CRG is composed by an interdisciplinary, motivated and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.

In April 2021, the Centre for Genomic Regulation (CRG) received the renewal of the 'HR Excellence in Research' Award from the European Commission.

This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).

Please, check out our Recruitment Policy

The role

About the lab
Tumors originate from normal cells that acquire tumor-initiating genetic events, such as translocations and somatic mutations. These genetic hits turn normal cells into pre-malignant cells, but do not lead to immediate tumor formation. For that, secondary genetic events as well as epigenetic hits are required.

We are an expanding lab that recently was awarded an ERC starting grant providing funds for the coming 5 years.

We aim to create a better understanding of how epigenetic tumor-associated changes arise in the context of non-Hodgkin lymphomas.

To that end, we aim to study the occurrence of these changes in healthy individuals as well as in pre-malignant cells in vitro (to be created by CRISPR/Cas9 genomic editing) and in vivo using single-cell technologies.

On top of that, we aim to define cell intrinsic mechanisms, such as enhancer activation and 3D chromatin interactions,that influence the occurrence of the observed changes.

Whom would we like to hire?

Professional experience

Must Have

Minimum of 3 years of handson experience in biomedical/biology related experimental work.

Desirable but not required/ Nice to have

Hands-on expertise in:

Epigenetic analyses (e.g. ChIPseq, ATACseq, bisulfite sequencing, chromatin conformation capture).
Culturing, in vitro differentiation and manipulation of primary cell types (preferably hematopoietic cells)
Cell transfection protocols, including generation of the required plasmids
Genomeediting strategies (preferably hematopoietic cells)
Generation of nextgeneration sequencing libraries in different experimental contexts
FACS sorting
Valid accreditation and recent handson experience in working with laboratory animals, especially mice

Education and training
At least 3 years of hands-on experience in biomedical/biology related experimental work, combined with any of the following:

A lab technician qualification
A PhD degree in Biology (or related disciplines)
A bachelor/master's degree in Biology (or related disciplines)

Languages

You are proficient in English

Technical skills
Hands-on experience in at least 3 of the following areas:

Basic molecular biology
NGS methods
CRISPR/Cas9based technologies
Mammalian cell culture, including transfection methods for (primary) cell systems
FACS sorting
Epigenetic analyses
Optimisation of complex protocols
Working with laboratory animals, especially mice

Competences

Highly developed organization skills
Excellent problemsolving skills
Motivation to learn new techniques
Enthusiasm and dedication to implement new protocols in the lab
Good communication skills
Capability to work on different projects simultaneously as part of a team and/or independently

The Offer - Working Conditions
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Contract duration: 2 years with a possibility to extend
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Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales

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Target start date: 01/02/2023

We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section.

We offer and
promote a diverse and inclusive environment and welcomes applicants regardless of age, disability, gender, nationality, ethnicity, religion, sexual orientation or gender identity.

The
CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.